菲律宾太阳网娱乐Fast Lysis Method

• BioFast Soil Genomic DNA Extraction Kit MORE
  Product Details：

  Kit Components

  Cat#	BSC21S1
  Components	50Tests
  SP Buffer	45ml
  菲律宾太阳网娱乐Lysis S Buffer	5ml
  DA Buffer	12.5ml
  Binding Buffer	35ml
  Wash Buffer	菲律宾太阳网娱乐30ml(add 45ml absolute ethanol before use)
  菲律宾太阳网娱乐Elution Buffer	15ml
  Grind Tube	50tubes
  Spin Column	50tubes
  Handbook	1copy

  Introduction

  菲律宾太阳网娱乐    The kit provides a very simple, fast and economic way for the isolation of PCR-ready genomic DNA from soil, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields genomic DNA less than 30minutes. It does not require expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform.

      At first, the soil sample is lysed by SP buffer and Lysis S Buffer in the grind tube. Designed for using with the lysis instruments from BIOER,soil is easily lysed within 40 s.Also manual operation can be chose with vortex generator within 5minutes.

      Then DNA in the sample is liberated. After centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.

  Product Features：

  Storage

  菲律宾太阳网娱乐All reagents, when stored properly, are stable for 18 months.

  Apparatus and Materials to Be Supplied by the User

  菲律宾太阳网娱乐*  Sterile 2.0ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000g              * Absolute ethanol

  Important notes

  • 1. Please add 45ml absolute ethanol to Wash Buffer and mix thoroughly before the first use.
  • 2. Lysis S Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37°C until the precipitate has fully dissolved.

  Experimental data:

  Analysis DNA

  • Absorbance analysis

  Get some DNA，diluted in a advisable factor with elution buffer. Survey the OD260，OD280 and OD320.

  菲律宾太阳网娱乐Expressions：concentration（μg/ml）＝50×OD260×dilution fact

  Target： 2.0≥OD260-320/ OD280-320≥1.7

  菲律宾太阳网娱乐Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

  • Agarose Gel Analysis

  0.8～1％ Agarose gel

  Real-time Taqman PCR detect

  
  

  Manual download